Phenyl Sepharose High Performance

• Cat#No#: Ph-426C
• Product Overview: Phenyl Sepharose High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
  Aromatic HIC resin designed for intermediate purification and polishing steps.
  Selectivity similar to Phenyl Sepharose 6 Fast Flow (low sub) but higher resolution due to the smaller 34 µm bead size.
  BioProcess resin supported for industrial applications and well-established in approved processes.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
• Description: Phenyl Sepharose High Performance and Butyl Sepharose High Performance are hydrophobic interaction chromatography (HIC) resins.
• Characteristic: High-resolution, high-capacity separations with high recovery.
  Reliable and reproducible.
  High chemical stability for effective CIP and sanitization.
  Available in laboratory and BioProcess scale quantities.
  Easy to scale up.
• Maximum operating pressure: Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column.
• Packing Column: Preferred packing solution: 10% to 20% ethanol.
  Resin slurry concentration: 50%.
  Packing pressure: 0.3 to 0.6 MPa (3 to 6 bar, 43.6 to 87.0 psi).
  Packing flow velocity: 200 to 300 cm/h.
• Matrix: cross-linked agarose, spherical
• Average particle size: ~34 µm
• Ligand: Phenyl
• Ligand density: Approx. 25 µmol phenyl/ml gel
• Recommended flow rate: ≤ 100 cm/h
• Recommended column height: 30 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 1 M Acetic acid, 30% Acetonitrile, 70% Ethanol, 30% Isopropanol, 6 M guanidine-hydrochloride, 8 M Urea, 3 M ammoniumsulfate, ionic detergents, non-ionic detergents, polar organic solvents, 2% sodium dodectyl sulfate.
• pH working range: 3–13
• CIP stability: 2–14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Removal of precipitated proteins: Wash the column with 4 bed volumes of 0.01 M NaOH at 40 cm/h, followed by 2 to 3 bed volumes of water.
  Removal of tightly bound hydrophobic proteins, lipoproteins and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol followed by 3 to 4 bed volumes of water. Alternatively, wash the column with detergent in a basic or acidic solution, for example, 0.5% nonionic detergent in 1 M acetic acid. Wash at a flow velocity of 40 cm/h. Remove residual detergent with 5 bed volumes of 70% ethanol followed by 3 to 4 bed volumes of water.
• Sanitization: Wash the column with 0.01 M NaOH at a flow velocity of approximately 40 cm/h, contact time 30 to 60 min.
• Pack size: 75 mL
• BioProcess resin: Yes
• Maximum flow velocity: 4.0 mL/min (1 mL), 20 mL/min (5 mL)
• Dimensions: 0.7 × 2.5 cm (1 mL)
• Column volume: 1 mL and 5 mL
• Column hardware pressure limit: 5 bar (0.5 MPa, 73 psi)