Plasminogen Removal Gel

• Cat#No#: Pl-378C
• Product Overview: Plasminogen Removal Gel is a resin designed for industrial removal of plasminogen and plasminogen activator, the target molecule being for example fibrinogen. It can also be used for purification of plasminogen.
  Plasminogen Removal Gel is based on a highly cross-linked 4% agarose matrix, which enables rapid processing of large sample volumes. The ligand, trans-4-(aminomethyl)cyclohexane carboxylic acid, is attached to the base matrix via a long, hydrophilic spacer arm to make it easily available for binding of the target molecule.
• Maximum operating pressure: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Matrix: 4% cross-linked agarose
• Swelling: Plasminogen Removal Gel is supplied pre-swollen in a buffered 20% ethanol solution.
• Particle Size: 45 μm-165 μm
• Average particle size: 90 µm
• Ligand: Trans-4-(aminomethyl)cyclohexanecarboxylic acid
• Ligand density: Approx. 11 μmol/ml drained medium
• Recommended flow rate: 150–250 cm/h, 25 cm bed height, 0.1 MPa, distilled water in XK 50 column.
• Recommended column height: 25 cm
• Chemical stability: Stable to commonly used aqueous buffers: 0,5 M NaOH, 6 M guanidine hydrochloride.
• pH working range: 3–13
• CIP stability: 2–14
• Storage: 4 to 30°C, in 20% Ethanol and 0.06 M Sodium Acetate
• Cleaning-in-place: Prolonged exposure (i.e., several days) to pH < 2 should be avoided due to a slow decomposition of the matrix at low pH. Strongly bound proteins can be removed with urea or guanidine hydrochloride.
• Sanitization: Generally, sodium hydroxide (0.1 to 1 M), alone or in combination with sodium chloride (0.5 to 3 M) or ethanol (20% to 70%), is an effective sanitization agent.
• Pack size: 1 L
• BioProcess resin: No