PreDictor Capto adhere ImpRes

• Cat#No#: Pr-296P
• Product Overview: PreDictor plates are disposable, 96-well filter plates for parallel screening of resins and chromatographic conditions such as binding, wash, and elution conditions. Different applications may require different amounts of resins in the wells. Data generated using PreDictor plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening of process conditions.
• Characteristic: Supports high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions using a 96-well plate format.
  Allows fast and efficient evaluation of parameters for binding/wash/elution conditions, and resin benchmarking.
  Results show good correlation with data obtained by column chromatography.
  Each well is prefilled with defined amounts of Capto adhere ImpRes.
  Can be used with centrifugation or vacuum, manually or in automated robotic systems.
  Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation.
• Metal ion capacity: 0.08 to 0.11 mmol Cl- /mL resin
• Matrix: highly cross-linked agarose
• Average particle size: 36 to 44 µm
• Chemical stability: All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C
• Cleaning-in-place: Precipitated hydrophobically bound proteins or lipoproteins: Wash with at least 3 column volumes of 1 M NaOH at a low flow velocity with reversed flow direction. Contact time at least 15 min.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction.
  Lipids and very hydrophobic proteins: Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment.
  Nucleic acids: Wash with 0.1 M acetate, pH 3 for 2 to 5 column volumes followed by buffer at neutral pH for 1 to 2 column volumes. Then wash with at least 3 column volumes 1 M NaOH at a low flow velocity with reversed flow direction. Contact time 15 min.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 hour is recommended. The CIP protocol for precipitated, hydrophobic bound proteins or lipoproteins removes bound contaminants and sanitizes the chromatography resin effectively.
• Pack size: 6 μl
• Maximum flow velocity: 300 cm/h
• Dimensions: 127.8 x 85.5 x 30.6 mm
• Functional group: Multimodal strong anion exchanger
• Number of wells: 96
• Material: Polypropylene (PP) and Polyethylene (PE)
• Resin suspensions in total volumes: 200 μl for 2 μl sedimented medium/well 500 μl for 6, 20, and 50 μl sedimented medium/well For PreDictor isotherm plates: – 500 μl for 50 μl sedimented medium/well – 200 μl for 20 μl sedimented medium/well – 500 μl for 8 μl sedimented medium/well – 375 μl for 6 μl sedimented medium/well – 250 μl for 4 μl sedimented medium/well – 125 μl for 2 μl sedimented medium/well.
• Centrifugation force recommended: 100 x g (Sample Dependent)-500 x g (Sample Dependent)
• Centrifugation force max.: 700 x g
• Operating temp. max.: 30°C
• Operating temp. min.: 4°C