PreDictor Capto adhere

• Cat#No#: Pr-295P
• Product Overview: PreDictor plates are disposable, 96-well filter plates for parallel screening of resins and chromatographic conditions such as binding, wash, and elution conditions. Screen different chromatography resins simultaneously using dedicated screening plates, or more thoroughly using plates containing a single resin. Different applications require different amounts of resins in the wells, and we offer a wide range of PreDictor plates containing different chromatography resins at different volumes. Data generated using PreDictor plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening of process conditions.
• Characteristic: Supports high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions using a 96-well plate format.
  Allows fast and efficient evaluation of parameters for binding/wash/elution conditions, and resin benchmarking.
  Results show good correlation with data obtained by column chromatography.
  Each well is prefilled with defined amounts of BioProcess chromatography resins.
  Can be used with centrifugation or vacuum, manually or in automated robotic systems.
  Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation.
• Maximum operating pressure:  600 cm/h at  0.3 MPa in a 1 m diameter column and 20 cm bed height (at 20°C using process buffers with the same viscosity as water
• Metal ion capacity: 0.09 to 0.12 mmol Cl- /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 75 μm
• Chemical stability: Stable to commonly used aqueous buffers, 1 M acetic acid, 1.0 M NaOH.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Autoclavable: 17 min at 121°C in 0.05 M phosphate buffer, pH 7, 10 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Precipitated hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction.
  Lipids and very hydrophobic proteins: Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment.
  Nucleic acids: Wash with 0.1 M acetate pH 3 for 2 to 5 column volumes followed by equilibration buffer at neutral pH for 1 to 2 column volumes and wash 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 hour is recommended. The CIP protocol for precipitated, hydrophobic bound proteins or lipoproteins removes bound contaminants and sanitizes the resin effectively.
• Pack size: 6 μl
• Dimensions: 127.8 x 85.5 x 30.6 mm
• Functional group: Multimodal strong anion exchanger
• Number of wells: 96
• Material: Polypropylene (PP) and Polyethylene (PE)
• Resin suspensions in total volumes: 200 μl for 2 μl sedimented medium/well 500 μl for 6, 20, and 50 μl sedimented medium/well For PreDictor isotherm plates: – 500 μl for 50 μl sedimented medium/well – 200 μl for 20 μl sedimented medium/well – 500 μl for 8 μl sedimented medium/well – 375 μl for 6 μl sedimented medium/well – 250 μl for 4 μl sedimented medium/well – 125 μl for 2 μl sedimented medium/well.
• Centrifugation force recommended: 100 x g (Sample Dependent)-500 x g (Sample Dependent)
• Centrifugation force max.: 700 x g
• Operating temp. max.: 30°C
• Operating temp. min.: 4°C