PreDictor Capto DeVirS

• Cat#No#: Pr-082P
• Product Overview: PreDictor plates are disposable 96-well filter plates prefilled with BioProcess chromatography resins. PreDictor plates support highthroughput process development (HTPD) by allowing parallel screening of chromatographic conditions. They can be used in automated workflows using robotic systems, or can be operated manually using multi-channel pipettes. Each well in a PreDictor plate is prefilled with a defined amount of chromatography resin.
• Description: Capto DeVirS is a chromatography medium that is designed to be used for the capture and intermediate stages of virus purification. The combination of high capacity with high flow rates and low backpressure reduces process cycle times and increases productivity. The Capto family range consists of modern process chromatography media designed for fast, efficient, and cost-effective purification that meets the stringent demands of modern large-scale biopharmaceutical and vaccine manufacturing processes.
• Characteristic: Support high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions.
  Allow fast and efficient evaluation of parameters for binding/wash/elution conditions, and resin benchmarking.
  Each is pre-filled with defined amounts of BioProcess chromatography resin PreDictor 96-well filter plates can be used with centrifugation or vacuum, manually, or in automated robotic systems.
  Reduced experimental time: the time-scale for performing screening experiments can be reduced from weeks to hours.
  Low sample consumption: new format significantly reduces sample consumption.
  Increased process understanding: the enlarged experimental space allows for a significant increase in process understanding.
• Matrix: Highly cross-linked agarose
• Average particle size: 75 μm
• Ligand density: 70 to 130 µmol S/ml drained gel
• Recommended flow rate: At least 600 cm/h in a 1 m diameter column with a 20 cm bed height at 20°C using process buffers with the same viscosity as water at < 3 bar (0.3 MPa).
• Recommended column height: 20 to 40 cm
• Chemical stability: All commonly used aqueous buffers, 0.1 M NaOH.
• pH working range: 6 to 14
• CIP stability: 7 to 13
• Temperature stability: 4°C to 30°C
• Storage: 4 to 8°C, 20% Ethanol
• Cleaning-in-place: We recommend CIP after each cycle with 1 M NaOH for at least 30 min in reversed flow.
• Sanitization: 0.5 to 1.0 M NaOH with a contact time of 1 h
• Pack size: 4 × 96-well filter plates
• Dimensions: 127.8 x 85.5 x 30.6 mm
• Column volume: 800 µL
• Functional group: Dextran sulfate
• Number of wells: 96
• Material: Polypropylene (PP) and Polyethylene (PE)
• Resin suspensions in total volumes: 200 μl for 2 μl sedimented resin/well 500 μl for 6, 20, and 50 μl sedimented resin/well For PreDictor isotherm plates: – 500 μl for 50 μl sedimented resin/well – 200 μl for 20 μl sedimented resin/well – 500 μl for 8 μl sedimented resin/well – 375 μl for 6 μl sedimented resin/well – 250 μl for 4 μl sedimented resin/well – 125 μl for 2 μl sedimented resin/well.
• Centrifugation force recommended: 100 x g (Sample Dependent)-500 x g (Sample Dependent)
• Centrifugation force max.: 700 x g
• Operating temp. max.: 30°C
• Operating temp. min.: 4°C