PreDictor RoboColumn IMAC Sepharose 6FF

• Cat#No#: Pr-116P
• Product Overview: His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
• Description: His MultiTrap FF is prepacked 96-well filter plates for high-throughput screening of histidine-tagged* proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose 6 Fast Flow (His MultiTrap FF) or Ni Sepharose High Performance (His MultiTrap HP). A protocol for His MultiTrap is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.
• Characteristic: Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* proteins.
  Increased convenience and consistency with prepacked 96-well plates.
  No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
  Use His MultiTrap 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
  Easy scale-up to HisTrap FF, HisPrep FF 16/10 or HisTrap HP prepacked columns.
  High chemical stability and high binding capacity up to 1 mg of histidine-tagged protein per well.
• Metal ion capacity: ~15 μmol Ni2+ /mL resin
• Average particle size: 90 μm
• Chemical stability: 0.01 M HCl or 0.1 M NaOH for one week at 40 ºC. 1 M NaOH or 70% acetic acid for 12 h. 2% SDS for 1 h. 30% 2-propanol for 30 min.
• pH working range: 2 to 14
• CIP stability: 3 to 12
• Storage: 4 - 30°C, 20% Ethanol
• Pack size: 4 × 96-well filter plates
• Dimensions: 127.8 × 85.5 × 30.6 mm
• Number of wells: 96
• Material: Polypropylene and polyethylene
• Centrifugation force recommended: 100 x g-500 x g
• Centrifugation force max.: 700 x g