Procainamide Sepharose 4 Fast Flow

• Cat#No#: Pr-379C
• Description: Procainamide Sepharose 4 Fast Flow is an affinity medium designed to purify butyrylcholinesterase, a plasma protein involved in the regeneration of synapses of the nervous system. Lack of butyrylcholinesterase increases sensitivity to succinyl choline exposure. There is also strong evidence that butyrylcholinesterase is the major detoxicating enzyme of cocaine.
• Characteristic: Long spacer arm makes ligand easily available for binding target molecules.
  Excellent scalability.
  Ideal for industrial purification of butyrylcholinesterase.
• Maximum operating pressure: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm.
• Ligand Coupling Method: Amide linkage
• Matrix: 4% cross-linked agarose
• Particle Size: 45 μm-165 μm
• Average particle size: 90 µm
• Ligand: Procainamide
• Ligand density: Approx. 23 μmol/ml drained medium.
• Coupling chemistry: Amide linkage
• Recommended flow rate: 150 to 250 cm/h, 25 cm bed height, 0.1 MPa distilled water in an XK 50 column.
• Recommended column height: 25 cm
• Chemical stability: Stable to commonly used aqueous buffers: 0,1 M NaOH, 6 M guanidine hydrochloride.
• pH working range: 2–12
• CIP stability: 1–14
• Cleaning-in-place: A general recommendation is to use a guanidine hydrochloride solution to remove precipitated or denatured substances. For hydrophobic-bound substances, a non-ionic detergent or ethanol is recommended. Different concentrations of sodium hydroxide can also be used, but ensure that you keep the contact time as short as possible at higher concentrations.
• Sanitization: Combinations of sodium hydroxide and 20% ethanol can be efficient for sanitization. Prolonged exposure to pH lower than 2 should be avoided as the matrix decomposes at low pH.
• Pack size: 100 mL
• BioProcess resin: No