| Product Information | |
| Cat#No# | IMAC-003R |
| Product Overview | Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights. |
| Form | 50% slurry in 20% ethanol, Ni-charged |
| Characteristic | Chelating ligand is iminodiacetic acid (IDA) Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems Stable across a wide pH range (1–14) Works under native or denaturing conditions Can be used for single-step purification from crude samples or contaminant removal |
| Capacity | 12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) |
| Maximum operating pressure | 109 psi (7.5 bar) |
| Average particle size | 45–90 μm (mean 60 μm) |
| Ligand | Iminodiacetic acid (IDA) |
| Recommended flow rate | ≤600 cm/hr at 25°C |
| Temperature stability | 4–40°C |
| Autoclavable | Yes (in 0.1 M sodium acetate) |
| Shelf life | ≥1 year at ambient temperature in 20% ethanol |
| Storage | 4°C or ambient |
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