Profinity IMAC Resin, uncharged

• Cat#No#: IMAC-004R
• Product Overview: Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.
• Form: 50% slurry in 20% ethanol, uncharged
• Characteristic: Chelating ligand is iminodiacetic acid (IDA)
  Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
  Stable across a wide pH range (1–14)
  Works under native or denaturing conditions
  Can be used for single-step purification from crude samples or contaminant removal
• Capacity: 12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein)
• Maximum operating pressure: 109 psi (7.5 bar)
• Average particle size: 45–90 μm (mean 60 μm)
• Ligand: Iminodiacetic acid (IDA)
• Recommended flow rate: ≤600 cm/hr at 25°C
• Temperature stability: 4–40°C
• Autoclavable: Yes (in 0.1 M sodium acetate)
• Shelf life: ≥1 year at ambient temperature in 20% ethanol
• Storage: ≥1 year at ambient temperature in 20% ethanol