Protein A Affinity Resin (Enhanced Performance)

• Product Overview: This column is a novel affinity medium synthesized by coupling alkali-tolerant Protein A to a high-rigidity agarose matrix. Its optimized pore structure enhances antibody binding capacity. It is suitable for capturing monoclonal antibodies or Fc-fusion proteins from large-scale cell culture supernatants, and polyclonal antibodies from ascites or plasma. It can be cleaned in place with 0.1–0.5 M NaOH, avoiding costly and corrosive cleaning agents. Compared with similar products, it has higher binding capacity, enabling smaller column volumes during process scale-up to reduce production costs.
• Matrix: High-rigidity agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 40–120 μm
• Ligand: Recombinant Alkali-resistant protein A (24 kDa)
• Dynamic binding capacity: ≥ 60 mg Human IgG / mL packed resin
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–12
• pH CIP range: 2–13
• Chemical stability: Stable in common aqueous solutions: 10 mM acetic acid, 0.1 M sodium citrate, 8 M urea, 6 M guanidine HCl, 30% isopropanol, 20% ethanol
• Maximum operating pressure: 0.5 MPa
• Maximum flow velocity: ~500 cm/h, < 2 bar
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Storage: 2–8 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Recombinant alkali-resistant protein A ligand, specific for IgG and Fc-fusion proteins.
  Alkali Stability: Compatible with 0.1–0.5 M NaOH for cleaning and sanitization.
  Stable Supply: Meets industrial production requirements.