Protein A Affinity Resin (High Capacity, Alkali Stable)

• Product Overview: This resin product is a novel affinity medium synthesized by coupling alkali-tolerant Protein A to a high-rigidity agarose matrix. Its optimized pore structure enhances antibody binding capacity. It is suitable for capturing monoclonal antibodies or Fc-fusion proteins from large-scale cell culture supernatants, as well as polyclonal antibodies from ascites or plasma. The medium can be cleaned in place (CIP) using 0.1–0.5 M NaOH, avoiding the use of expensive and corrosive cleaning agents, effectively reducing costs. Compared with similar products, it has higher binding capacity, allowing smaller column volumes during process scale-up to lower production costs.
• Matrix: High-rigidity agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 40–100 μm
• Ligand: Recombinant Alkali-resistant Protein A
• Dynamic binding capacity: ≥ 75 mg Human IgG / mL resin (contact time 6 min)
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–12
• pH CIP range: 2–13
• Chemical stability: Stable in common aqueous solutions: 10 mM acetic acid, 0.1 M sodium citrate, 8 M urea, 6 M guanidine HCl, 30% isopropanol, 20% ethanol
• Maximum operating pressure: 0.5 MPa
• Maximum flow velocity: ≥ 300 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
• Storage: 2–8 °C in 20% ethanol or 2% benzyl alcohol
• Product features: High Capacity: ≥ 75 mg/mL, significantly improving purification efficiency.
  High Pressure Resistance: High-rigidity agarose matrix with 60 μm particle size, tolerates up to 5 bar pressure.
  Low back pressure: < 1 bar under ID=1600 mm, H=25 cm conditions.
  High Alkali Resistance: Ligand stable to 0.1–0.5 M NaOH during CIP cleaning.
  Robust Scalability: Consistent batch-to-batch performance ensures product quality and continuous production.