Protein A Affinity Resin (High Stability)

• Product Overview: This resin product is a next-generation, highly alkali-tolerant Protein A affinity medium. The Protein A ligand is expressed in E. coli. Compared with similar products, it provides stronger alkali tolerance (CIP with 0.5–1.0 M NaOH) and higher binding capacity. Smaller column volumes can be used during process scale-up to reduce production costs. It is suitable for capturing monoclonal antibodies or Fc-fusion proteins from large-scale cell culture supernatants, and polyclonal antibodies from ascites or plasma.
• Matrix: High-rigidity agarose
• Appearance: White paste, may separate upon standing
• Average particle size: ~60 μm
• Ligand: Recombinant Alkali-resistant protein A
• Dynamic binding capacity: ≥ 75 mg Human IgG / mL packed resin (contact time 6 min)
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–12
• pH CIP range: 2–14
• Chemical stability: Stable in common aqueous solutions: 10 mM HCl, 0.1 M citric acid (pH 3), 6 M urea, 6 M guanidine HCl, 30% isopropanol, 20% ethanol
• Maximum operating pressure: 0.5 MPa
• Maximum flow velocity: ≥ 300 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
• Storage: 2–8 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Recombinant alkali-resistant protein A ligand, specific for IgG and Fc-fusion proteins.
  Alkali Stability: Compatible with 0.1–0.5 M NaOH for cleaning and sanitization.
  High Binding Capacity: Suitable for large-scale high-density bioreactor processing.
  Reliable Supply: Industrial-grade product consistency.