Protein A Affinity Resin

• Product Overview: This resin product is a novel affinity chromatography medium prepared by coupling recombinant alkali-tolerant Protein A ligands to a high-rigidity agarose matrix. Compared with similar products, the ligand is more stable, allows higher flow rates, and generates lower back pressure. It is suitable for capturing monoclonal antibodies or Fc-fusion proteins from large-scale cell culture supernatants, as well as polyclonal antibodies from ascites or plasma. The medium can be cleaned in place with 0.1–0.5 M NaOH, avoiding expensive and corrosive reagents, and is suitable for industrial-scale production.
• Matrix: High-rigidity agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 40–120 μm
• Ligand: Recombinant Alkali-resistant protein A (24 kDa)
• Dynamic binding capacity: ≥ 40 mg Human IgG / mL packed resin
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–12
• pH CIP range: 2–13
• Chemical stability: Stable in common aqueous solutions: 10 mM acetic acid, 0.1 M sodium citrate, 8 M urea, 6 M guanidine HCl, 30% isopropanol, 20% ethanol
• Maximum operating pressure: 0.5 MPa
• Maximum flow velocity: ~500 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Storage: 2–8 °C in 20% ethanol or 2% benzyl alcohol
• Product features: High-capacity alkali-resistant Protein A affinity resin.
  High-rigidity agarose matrix with optimized pore structure for antibody binding.
  Suitable for capturing monoclonal antibodies or Fc-fusion proteins from cell culture supernatants.