Product Information | |
Cat#No# | HP-046P |
Product Overview | Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species. |
Description | Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein. |
Characteristic | Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use. Flexible format, with protocols for classic elution and cross-link method. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provides high yield. Easy scale-up with HiTrap Protein A HP prepacked columns. Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems. |
Applications | Centrifugation ; Vacuum |
Ligand Coupling Method | N-hydroxysuccinimide activation |
Matrix | Highly cross-linked agarose, 6% |
Average particle size | 34 μm |
Ligand | Protein A |
Ligand density | Approx. 3 mg protein A/mL medium |
Dynamic binding capacity | > 10 mg human IgG/mL medium |
pH working range | 3–9 |
CIP stability | 2–9 |
Temperature stability | 4°C to 30°C |
Storage | 2 - 8°C, 20% Ethanol |
Shipping | 20% ethanol |
Pack size | 4 |
Dimensions | 127.8 × 85.5 × 30.6 mm |
Column volume | 800 μL |
Material | Polypropylene and polyethylene |
Resin suspensions in total volumes | 50 μl |
Centrifugation force recommended | <700 x g |
Operating temp. max. | 30°C |
Operating temp. min. | 4°C |
Scale | Lab |
Format | Prepacked 96-well plate |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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