Protein A HP MultiTrap

• Cat#No#: Pr-192P
• Product Overview: Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species.
• Description: Protein A HP MultiTrap is designed for the enrichment of proteins of interest, immunoprecipitation, and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Protein A HP MultiTrap are prepacked 96-well filter plates. Both products contain Protein A Sepharose High Performance.
  The spin columns and 96-well filter plates address the need for flexible, small-scale preparation of protein samples before downstream protein analyses such as gel electrophoresis, liquid chromatography, and LC-MS. When using the columns and plates for protein enrichment, an antibody of choice is first immobilized onto the Protein A media, and then used for the capture and enrichment of the protein of interest. The protein can be enriched several hundredfold, depending on the specificity of the antibody.
• Characteristic: Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  Flexible format, with protocols for classic elution and cross-link method.
  Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  Fast binding kinetics and high capacity provides high yield.
  Easy scale-up with HiTrap Protein A HP prepacked columns.
  Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses.
  MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
• Applications: Centrifugation ; Vacuum
• Ligand Coupling Method: N-hydroxysuccinimide activation
• Matrix: Highly cross-linked agarose, 6%
• Average particle size: 34 μm
• Ligand: Protein A
• Ligand density: Approx. 3 mg protein A/mL medium
• Dynamic binding capacity: > 10 mg human IgG/mL medium
• pH working range: 3–9
• CIP stability: 2–9
• Storage: 2 - 8°C, 20% Ethanol
• Pack size: 4
• Dimensions: 127.8 × 85.5 × 30.6 mm
• Material: Polypropylene and polyethylene
• Resin suspensions in total volumes: 50 μl
• Centrifugation force recommended: <700 x g
• Operating temp. max.: 30°C
• Operating temp. min.: 4°C
• Scale: Lab
• Format: Prepacked 96-well plate