Protein A HP SpinTrap

• Cat#No#: HP-047P
• Product Overview: Protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain Protein A Sepharose HP.
• Description: Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
• Characteristic: For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use.
  Classic and cross-link protocols provide flexibility.
  Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  Fast binding kinetics and high capacity provide high yield.
  Prepacked* with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses.
  Easy scale-up with HiTrap Protein A HP prepacked columns.
• Applications: Enrichment of target proteins Purification of antibodies.
• Sample preparation: 600 μl
• Ligand Coupling Method: N-hydroxysuccinimide activation
• Matrix: Highly cross-linked agarose, 6%
• Average particle size: 34 μm
• Ligand: Native protein A
• Ligand density: Approx. 3 mg protein A/mL medium
• Dynamic binding capacity: > 1 mg human IgG well
• pH working range: 3–9
• CIP stability: 2–9
• Temperature stability: 4°C to 30°C
• Storage: 2 - 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Pack size: 16 columns
• Dimensions: 127.8 × 85.5 × 30.6 mm
• Column volume: 800 μL
• Material: Polypropylene and polyethylene