Protein G Affinity Resin

• Product Overview: This affinity medium is prepared by immobilizing Protein G onto the 4% cross-linked agarose matrix. Protein G has a broader binding spectrum than Protein A, binding strongly to the Fc region and weakly to the Fab region. It is commonly used for isolating and purifying antibodies or antibody fragments from cell culture supernatants, as well as antibodies from sera of various species.
• Matrix: 4% Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 45–165 μm
• Ligand: Protein G (E. coli)
• Dynamic binding capacity: ~20 mg Human IgG / mL packed resin
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–12
• Chemical stability: 40 °C 1 week: 1 M acetic acid (pH 2.0), 20 mM sodium phosphate, 1% SDS, 6 M guanidine HCl, 70% ethanol
• Maximum operating pressure: 0.3 MPa
• Maximum flow velocity: 150–250 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
• Product features: Recombinant Protein G ligand, specific for IgG, Fc, and Fab fragments.
  Complementary to Protein A Resins: Favorable binding for rat IgG, human IgG3, and mouse IgG1.
  Applications: Purification from upstream feedstocks or serum of various species.