Protein G GraviTrap antibody purification columns

• Cat#No#: Pr-049P
• Product Overview: Protein G GraviTrap includes ten columns with 1 mL of resin for general purpose purification of polyclonal and monoclonal antibodies from most species.
• Description: Protein G GraviTrap is prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
• Characteristic: Broad binding specificity – binds a broad range of IgG species and subclasses from most species including rat.
  High binding capacity – approximately 20 mg human IgG/mL resin.
  Simple – GraviTrap prepacked gravity flow columns enable simple one-step purification of proteins in 30 min without the need for equipment.
• Applications: Fast and efficient manual purification of monoclonal and polyclonal antibodies.
• Matrix: Highly cross-linked agarose, 4%
• Average particle size: 90 µm
• Ligand: Recombinant Protein A and Protein G
• Dynamic binding capacity: Approx. 20 mg human IgG/column
• Temperature stability: Room temperature
• Storage: 2-8°C, 20% Ethanol
• Shipping: 20% ethanol
• Wash buffer: 20 mM sodium phosphate, pH 7.0
• Column volume: 1 ml
• Material: Polypropylene (PP) barrel, Polyethylene (PE) frits