Protein G HP SpinTrap

• Cat#No#: Pr-050P
• Product Overview: Protein G HP SpinTrap is a prepacked, single-use spin column containing Protein G Sepharose High Performance.
• Description: Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).
• Characteristic: For simple, small-scale purification of antibodies or enrichment of specific proteins in prepacked spin columns that are ready to use. Classic and cross-link protocols provide flexibility.
  Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  Fast binding kinetics and high capacity provides high yield.
  Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  Easy scale-up with HiTrap Protein G HP prepacked columns.
• Applications: Designed for small-scale sample preparation for single use: Enrichment of target protein Protein-protein interaction studies Upstream of gel electrophoresis, liquid chromatography and mass spectrometry.
• Sample preparation: 600 μl
• Ligand Coupling Method: N-hydroxysuccinimide activation
• Matrix: Highly cross-linked agarose, 6%
• Average particle size: 34 µm
• Ligand: Recombinant protein G lacking albumin-binding region.
• Ligand density: Approx. 2 mg protein G/mL medium
• Dynamic binding capacity: > 1 mg human IgG/column
• pH working range: 3–9
• CIP stability: 2–9
• Temperature stability: 4°C to 30°C
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Pack size: 16 columns
• Dimensions: 127.8 × 85.5 × 30.6 mm
• Column volume: 800 µL
• Material: Polypropylene and polyethylene