Protein G Sepharose 4 Fast Flow resin

• Cat#No#: Pr-434C
• Product Overview: Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.
• Characteristic: High binding capacity: approximately 20 mg human IgG per mL resin.
  Broad-spectrum: binds to a broad range of IgG species and subclasses.
  Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses.
  No specific albumin binding: recombinant protein G has been genetically engineered to remove the albumin binding region.
  Minimal leaching: optimized coupling method minimizes leaching of recombinant protein G and improves binding capacity for IgG.
  Wide range of formats: including loose resin packs from 5 mL to 5 L, for manual or automated antibody purification and immunoprecipitation procedures.
• Maximum operating pressure: Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water).
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: cross-linked agarose, 4%, spherical
• Average particle size: ~90 µm
• Ligand: Recombinant protein G lacking albumin-binding region, produced in E. coli.
• Ligand density: 2 mg Protein G/ml medium
• Dynamic binding capacity: ≥ 20 mg human IgG/mL resin
• Recommended flow rate: 150-250 cm/h
• Recommended column height: 25 cm
• Chemical stability: Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitane digluconate.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–9
• CIP stability: 2–10
• Temperature stability: 2˚C to 40˚C
• Storage: 2 to 8°C, 20% Ethanol
• Cleaning-in-place: Remove strongly bound hydrophobic proteins, lipoproteins and lipids by washing the column with a nonionic detergent, 0.1%, at 37°C, contact time one minute. Immediately reequilibrate with at least 5 bed volumes of sterile filtered binding buffer.
  Alternatively, wash the column with 70% ethanol and let stand for 12 hours. Re-equilibrate with at least 5 bed volumes of sterile binding buffer.
• Sanitization: Sanitization reduces microbial contamination of the chromatography resin to a minimum.
  Wash the column with a buffer containing 2% hibitane digluconate and 20% ethanol. Allow to stand for 6 hours.
• Pack size: 5 mL
• BioProcess resin: Yes
• Dimensions: 5 cm