Compared with Coomassie Brilliant Blue G-250, Coomassie Brilliant Blue R-250 has relatively weaker hydrophobicity and a relatively lower binding speed with proteins. This reagent adopts the ion pair method, which makes Coomassie Brilliant Blue R-250 bind to protein faster, but the degree of binding to glue is lower, so the decolorization time is greatly reduced, and it does not need to be fixed, which can be about 1 h Complete, the minimum 10 ng protein band can be detected.