PureBind Co-NTA Agarose 6 Fast Flow

• Cat#No#: AC-007I
• Product Overview: Co-NTA Agarose 6FF is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.
• Characteristic: Mechanical stable, suited for preparative and large scale purification of histidine-tagged proteins
  Higher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.
  Suitable for purifying proteins from prokaryotic and eukaryotic expression systems
  Compatible with applications under native and denaturing conditions
  Low cobalt ion leakage.
• Matrix: Highly cross-linked 6% agarose resins supplied as 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Cobalt ion
• Dynamic binding capacity: >20 mg 6XHis-tagged protein/mL medium
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical compatibility: (1) Reducing agents
  10 mM β-mercaptoethanol
  (2) Denaturing agents
  8 M urea, 6 M Gua-HCl
  (3) Detergents
  <1% Triton™ X-100 (nonionic)
  1% NP-40 (nonionic)
  1% CHAPS (zwitterionic)
  SDS
  sarcosyl
  (4) Other additives
  ≤500 mM imidazole at pH7.0 ~ 8.0 for elution
  30% ethanol
  20% glycerol
  500 mM KCl
  1.0 M NaCl
  20 mM MES
  50 mM Tris
  50 mM HEPES
  60 mM MOPS
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol