PureBind Co-NTA Agarose

• Cat#No#: AC-009I
• Product Overview: PureBind Co-NTA Agarose is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Ni-NTA media.
• Characteristic: Suited for batch or gravity flow purification of histidine-tagged proteins
  Higher specificity and selectivity for His-tagged proteins, compared with Ni-NTA IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.
  Suitable for purifying proteins from prokaryotic and eukaryotic expression systems
  Compatible with applications under native and denaturing conditions
  Low cobalt ion leakage.
• Applications: Co-NTA Agarose resins can be used for batch/gravity flow purification of polyhistidine-tagged recombinant proteins.
• Matrix: 4% agarose resins supplied as 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Cobalt ion
• Dynamic binding capacity: >20 mg 6XHis-tagged protein/mL medium
• Maximum Pressure Drop: 0.1 MPa (1 bar)
• Chemical compatibility: (1) Reducing agents: 10 mM β-mercaptoethanol
  (2) Denaturing agents: 8 M urea, 6 M Gua-HCl
  (3) Detergents:
  <1% Triton™ X-100 (nonionic)
  1% NP-40 (nonionic)
  1% CHAPS (zwitterionic)
  SDS
  sarcosyl
  (4)Other additives:
  ≤500 mM imidazoleat pH7.0 ~ 8.0 for elution
  30% ethanol
  20% glycerol
  500 mM KCl
  1.0 M NaCl
  20 mM MES
  50 mM Tris
  50 mM HEPES
  60 mM MOPS
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol