Q Sepharose Big Beads

• Cat#No#: Q -454C
• Product Overview: Q Sepharose Big Beads is a strong anion exchanger designed for industrial applications in the capture step of large volumes of feed or viscous feedstocks.
• Description: Q Sepharose Big Beads is composed of large crosslinked agarose beads (100-300 µm) modified with quaternary ammonium (Q) strong anion exchange groups. It is designed for industrial applications and the large particle size and physical stability of the base matrix ensure maintained performance and low back-pressures even with viscous samples.
• Characteristic: Strong anion exchanger for capture steps handling large volumes of feed.
  Large agarose beads (100-300 µm) permit capture from viscous feedstocks at high flow velocities.
  High chemical resistance for effective cleaning-in-place (CIP).
  BioProcess resin supported for industrial applications and well-established in approved processes.
• Maximum operating pressure: 1200-1800 cm/h, 100 kPa, XK 50/60 column.
• Ligand Coupling Method: Ether bonds
• Metal ion capacity: 0.18-0.25 mmol Cl-/ml medium
• Packing Column: Q Sepharose Big Beads is easy to pack in small and large scale columns. Narrow peaks with high symmetry are reproducible whether you pack the ion exchanger bed with a constant pressure of between 1 to 3 bar, or let the slurry sediment and then compress it with the adaptor. Suction packing can easily be performed as well.
• Matrix: 6% cross-linked agarose
• Ionic Exchanger Type: Strong anion exchanger
• Particle Size: 100 µm-300 µm
• Average particle size: ~200 µm
• Ligand: Quaternary amine
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqeous buffers, 1M NaOH, 70% ethanol, organic solvents.
• pH working range: 2–12
• CIP stability: 2–14
• Temperature stability: 4 to 30˚C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Cleaning-in-place Ionically bound proteins: Wash with filtered 2 M NaCl at approximately 100 cm/h. Contact time: 10 to 15 min.
  Hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h. Contact time: 1–2 h.
  Lipids and very hydrophobic proteins: Wash with 70% ethanol at 40 cm/h, reversed flow, or with saw-tooth gradient 0–30–0% isopropanol. Contact time: 1–2 h.
• Sanitization: A reduction of microbial contamination in the ion exchanger bed is obtained by washing the column with 0.5–1.0 M NaOH, allowing a contact time of 30–60 min.
• Pack size: 1 L
• BioProcess resin: Yes