Q Sepharose Fast Flow

• Cat#No#: Q -455C
• Product Overview: Q Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
• Description: Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups.
• Characteristic: Well-proven strong anion exchanger developed for industrial downstream processes.
  Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
  The industry standard for ion exchange chromatography during recent decades.
  High chemical stability allows for well proven CIP and sanitization protocols.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
• Maximum operating pressure: 400-700 cm/h, 100 kPa, XK 50/30 column
• Metal ion capacity: 0.18-0.24 mmol Cl-/ml medium
• Matrix: cross-linked, 6% agarose particles
• Ionic Exchanger Type: Strong anion exchanger
• Average particle size: ~90 µm
• Ligand: Quaternary amine
• Dynamic binding capacity: ~ 42 mg BSA/mL resin
• Recommended flow rate: 400 to 700 cm/h
• Recommended column height: 15 cm
• Chemical stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol.
• pH working range: 2–12
• CIP stability: 2–14
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 700 cm/h
• Functional group: -N+ (CH3 ) 3