Q Sepharose XL

• Cat#No#: Q -456C
• Product Overview: Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
• Description: Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow resin. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
• Characteristic: High loading capacities give more productive capture from clarified feedstocks.
  Strong anion exchanger designed for capture steps.
  High chemical stability for effective CIP/sanitization.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived. impurities in the elution pool.
• Ligand Coupling Method: Dextran extenders through ether bonds.
• Metal ion capacity: 0.18-0.26 mmol Cl-/ml medium.
• Matrix: 6% cross-linked agarose with dextran surface extenders.
• Ionic Exchanger Type: Strong anion exchanger
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 μm
• Ligand: Quaternary amine
• Dynamic binding capacity: ≥ 160 mg BSA/mL resin
• Recommended flow rate: 300-500 cm/h
• Chemical stability: Commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride.
• pH working range: 2–12
• CIP stability: 2–14
• Temperature stability: 4˚C to 30˚C
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 300 mL
• BioProcess resin: Yes
• Maximum flow velocity: 500 cm/h