ReadyToProcess Butyl Sepharose HP

• Cat#No#: Re-185P
• Product Overview: ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These ReadyToProcess columns are prepacked with Butyl Sepharose High Performance chromatography resin and are used for the intermediate stages of protein purification when high resolution has priority.
• Description: Butyl Sepharose High Performance is based on highly cross-linked, 34 µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. The resin is truly hydrophobic, displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well-suited for intermediate purification and polishing steps, providing high resolution due to the small particle size.
• Characteristic: Aliphatic HIC resin designed for intermediate purification and polishing steps.
  Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size.
  Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations.
  BioProcess resin supported for industrial applications.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 2.4 bar (0.24 MPa, 35 psi)
• Recommended column height: 200 mm
• Storage: 4°C to 30°C, 20% ethanol
• Pack size: 5 L
• Dimensions: 178 × 200 mm
• Column volume: 5 L
• Column i.d.: 178 mm