ReadyToProcess Capto Butyl ImpRes columns

• Cat#No#: Re-429C
• Product Overview: These chromatography columns are prepacked with Capto Butyl ImpRes hydrophobic interaction chromatography (HIC) resin. The columns are an excellent choice for intermediate and polishing steps in downstream purification processes.
  Prepacked and ready-to-use: validated high-performance columns supplied prepacked and ready-for-use in bioprocessing.
  High-resolution purification: based on Capto ImpRes base matrix with a traditional HIC ligand and a small average particle size. Flexible process design: large operational window of flow velocities and bed heights.
  Higher throughput compared with Capto Butyl: enables improved productivity and process economy.
  Excellent chemical stability.
  Resin fulfills industrial demands: for security of supply, robust performance, and regulatory support.
• Description: ReadyToProcess chromatography columns are validated highperformance bioprocessing columns that are supplied prepacked and ready for use. The columns are available with a range of BioProcess resins in different sizes. Standardized column formats allow off-the-shelf availability for short delivery lead times. ReadyToProcess columns are closed units and the design allows easy disposal after completed production, enabling flexibility in operations.
• Characteristic: Standardized column formats allow reduced column cost and off-the-shelf availability for short delivery lead times.
  Robust column design and validated automated packing methods enable consistent column performance.
  Cost-savings by eliminating the need for column preparation, packing, and validation procedures.
  Customer safety stock possibility for security of supply.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I and II studies. Depending on the scale of operations, the columns can also be used for full-scale manufacturing, as well as for preclinical studies.
• Recommended column height: 200 mm
• Storage: 4°C to 30°C, 20% ethanol
• Sanitization: Although most ReadyToProcess columns are delivered presanitized, the column design allows for easy sanitization. The sanitization procedure reduces both endotoxins and microorganisms, including spore-forming organisms. Each sanitized column is sampled for endotoxin analysis (acceptance limit < 0.25 EU/mL) and microbial growth (colony forming units < 10 CFU/100 mL). The results, including results from efficiency testing as well as endotoxin and microbial analyses, are presented in the CoA. As a last step, the column is equilibrated in 20% ethanol (20% ethanol with 0.2 M sodium acetate, pH 5.5 when applicable).
  All resins are not sodium hydroxide-stable, such as some of the affinity chromatography resins. Hence, columns containing such resins are delivered non-sanitized. Microbial analyses are included in the CoA (acceptance limit < 100 CFU/mL). Non-sanitized columns have NS included in the end of the product description.
• Pack size: 10 L
• Maximum operating backpressure: 0.3 MPa (3 bar, 44 psi)
• Dimensions: 251 × 200 mm
• Column volume: 10 L
• Column i.d.: 251 mm