ReadyToProcess Capto Core 400

• Cat#No#: Re-301P
• Product Overview: ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These single-use chromatography columns are prepacked with Capto Core 400 resin for purification of large entities such as viruses and viral vectors. Capto Core 400 resin is based on core-bead technology, enabling combination of size exclusion chromatography and binding chromatography for effective and productive separation.
• Description: Capto Core 400 is composed of a ligand-activated core and an inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 400 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough, while smaller impurities bind to the internalized ligands. Capto Core 400 is an excellent alternative to the size exclusion chromatography resins typically employed in the final stages of virus purification in vaccine manufacture.
• Characteristic: Novel core bead technology and multimodal, octylamine ligand give Capto Core 400 dual functionality with both size exclusion and binding properties.
  Efficient capture of contaminants while target molecules are collected in the flowthrough.
  Significantly improved productivity and higher flow rates compared with standard size exclusion chromatography (gel filtration) methods.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, the column can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 3 bar (0.3 MPa, 44 psi)
• Matrix: Highly cross‑linked agarose
• Average particle size: 90 μm
• Ligand: Octylamine
• Dynamic binding capacity: 22 mg ovalbumin/mL resin
• Recommended column height: 200 mm (7.87 in)
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide (NaOH), 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 3 to 14
• Storage: 4˚C to 30˚C, 20% ethanol
• Cleaning-in-place: Use of 1 M NaOH in 27% 1‑propanol is recommended for effective CIP and sanitization of the resin after every cycle.
• Pack size: 32 L
• Maximum flow velocity: 700 cm/h in column with 20 cm bed height at < 2 bar (0.2 MPa).
• Dimensions: 450 × 200 mm
• Column volume: 32 L
• Column i.d.: 450 mm