ReadyToProcess Capto Core 700

• Cat#No#: Re-302P
• Product Overview: ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These ReadyToProcess columns are prepacked with Capto Core 700 chromatography resin for purification of large entities such as viruses and viral vectors.
• Description: Capto Core 700 is composed of a ligand-activated core and an inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough, while smaller impurities bind to the internalized ligands.
• Characteristic: Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality.
  This chromatography resin has both size exclusion and binding properties.
  Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough. Significantly improved productivity and higher flow rates compared with standard size exclusion (gel filtration) methods. Straightforward optimization due to flowthrough chromatography and robust performance.
  Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 2 bar (0.2 MPa, 29 psi)
• Matrix: Highly cross‑linked agarose
• Average particle size: 85 μm
• Dynamic binding capacity: 13 mg ovalbumin/mL resin
• Recommended column height: 200 mm (7.87 in)
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide (NaOH), 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 3 to 14
• Storage: 4°C to 30°C, 20% ethanol
• Cleaning-in-place: Use of 1 M NaOH in 27% 1‑propanol is recommended for effective CIP and sanitization of the resin after every cycle.
• Maximum flow velocity: 500 cm/h in column with 20 cm bed height at < 2 bar (0.2 MPa)
• Dimensions: 80 × 200 mm
• Column volume: 1 L
• Column i.d.: 80 mm