| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Re-089P | ReadyToProcess IMAC Sepharose 6 Fast Flow columns | Inquiry |
| Product Information | |
| Cat#No# | Re-089P |
| Product Overview | Chromatography columns prepacked with IMAC Sepharose 6 Fast Flow, an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions. |
| Description | Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein. |
| Characteristic | Prepacked and ready-to-use: validated high-performance columns supplied prepacked and ready for use in bioprocessing. Supplied uncharged: for customized metal ion charging and optimized selectivity. For purification of histidine-tagged (his-tagged) proteins: select when nickel has been shown to not be the best choice of metal ion. High chemical stability: enables proven cleaning-in-place and sanitization protocols. Hydrophilic base matrix: ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Resin fulfills industrial demands: for security of supply, robust performance, and regulatory support. |
| Maximum operating pressure | 1.2 bar (0.12 MPa, 17 psi) |
| Metal ion capacity | Approx. 15 µmol Ni2+/mL medium |
| Matrix | Highly cross-linked 6% spherical agarose |
| Average particle size | 90 µm |
| Dynamic binding capacity | (Histidine)6 -tagged: Approx. 40 mg/mL medium (Ni2+-charged) Untagged: Approx. 25 mg/mL medium (Cu2+-charged), approx. 15 mg/mL medium (Zn2+ or Ni2+-charged) |
| Recommended column height | 200 mm (7.87 in) |
| Chemical stability | 0.01 M HCl, 0.1 M NaOH. Tested for 1 week at 40°C. 1 M NaOH, 70% acetic acid. Tested for 12 h. 2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min. |
| pH working range | 3 to 12 |
| CIP stability | 2 to 14 |
| Storage | 4˚C to 30˚C, 20% ethanol |
| Pack size | 1 L |
| Maximum flow velocity | 600 cm/h (20 mL/min) using XK 16/20 columns with 5 cm bed height. |
| Dimensions | 80 × 200 mm |
| Column volume | 1 L |
| Column i.d. | 80 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa, 72 psi) |
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For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
