ReadyToProcess Sepharose 6 FF
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ReadyToProcess Sepharose 6 FF

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Re-317P ReadyToProcess Sepharose 6 FF Inquiry
Product Information
Cat#No# Re-317P
Product Overview ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These ReadyToProcess columns are prepacked with Sepharose 6 Fast Flow chromatography resin for industrial processing at high flow rates and moderate pressures.
Description Sepharose 6 Fast Flow size exclusion chromatography resin is based on cross-linked 6% agarose matrix, which gives good physical stability and chromatographic qualities. The matrix was developed specifically to meet the high-throughput demands of industrial process separations. The rigidity permits high flow rates, which in turn gives good resolution in a minimum amount of time. Sepharose Fast Flow matrices enable the rapid processing of large volumes making these resins an excellent choice for use at process scale.
Characteristic Industrial-scale separation of large molecules and virus particles.
High flow velocities at moderate pressures deliver good resolution with speed.
Low non-specific interactions and excellent recoveries.
Applications For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
Maximum operating pressure 1.2 bar (0.12 MPa, 17 psi)
Matrix Cross-linked agarose, 6%, spherical
Average particle size ~ 90 µm
Recommended column height 200 mm
Chemical stability Stable to commonly used aqueous buffers, 2.0 M NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 2% asepto, 6 M guanidine hydrochloride, 8 M urea.
pH working range 3 to 13
CIP stability 2 to 14
Autoclavable 20 min at 121°C in H2 O
Storage 4°C to 30°C, 20% ethanol
Cleaning-in-place Remove precipitated proteins: Wash the column with 4 bed volumes of 1.0 to 2.0 M NaOH solution at 40 cm/h, followed by 2 to 3 bed volumes of water.
Remove strongly bound hydrophobic proteins, lipoproteins, and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol (apply gradients to avoid air bubble formation when using high concentrations of organic solvents). Alternatively, wash the column with detergent in a basic or acidic solution. For example, use 0.5% nonionic detergent in 1.0 M acetic acid. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
Sanitization Sanitization using NaOH reduces microbial contamination of the resin bed to a minimum without dismantling the column. The CIP procedures recommended above also sanitize Sepharose Fast Flow resins effectively. A concentration of 1.0 to 2.0 M NaOH with a contact time of 30 to 60 min has proved effective for most microbial contamination.
Pack size 2.5 L
Dimensions 126 × 200 mm
Column volume 2.5 L
Column i.d. 126 mm
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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