Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Re-317P | ReadyToProcess Sepharose 6 FF | Inquiry |
Product Information | |
Cat#No# | Re-317P |
Product Overview | ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These ReadyToProcess columns are prepacked with Sepharose 6 Fast Flow chromatography resin for industrial processing at high flow rates and moderate pressures. |
Description | Sepharose 6 Fast Flow size exclusion chromatography resin is based on cross-linked 6% agarose matrix, which gives good physical stability and chromatographic qualities. The matrix was developed specifically to meet the high-throughput demands of industrial process separations. The rigidity permits high flow rates, which in turn gives good resolution in a minimum amount of time. Sepharose Fast Flow matrices enable the rapid processing of large volumes making these resins an excellent choice for use at process scale. |
Characteristic | Industrial-scale separation of large molecules and virus particles. High flow velocities at moderate pressures deliver good resolution with speed. Low non-specific interactions and excellent recoveries. |
Applications | For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies. |
Maximum operating pressure | 1.2 bar (0.12 MPa, 17 psi) |
Matrix | Cross-linked agarose, 6%, spherical |
Average particle size | ~ 90 µm |
Recommended column height | 200 mm |
Chemical stability | Stable to commonly used aqueous buffers, 2.0 M NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 2% asepto, 6 M guanidine hydrochloride, 8 M urea. |
pH working range | 3 to 13 |
CIP stability | 2 to 14 |
Autoclavable | 20 min at 121°C in H2 O |
Storage | 4°C to 30°C, 20% ethanol |
Cleaning-in-place | Remove precipitated proteins: Wash the column with 4 bed volumes of 1.0 to 2.0 M NaOH solution at 40 cm/h, followed by 2 to 3 bed volumes of water. Remove strongly bound hydrophobic proteins, lipoproteins, and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol (apply gradients to avoid air bubble formation when using high concentrations of organic solvents). Alternatively, wash the column with detergent in a basic or acidic solution. For example, use 0.5% nonionic detergent in 1.0 M acetic acid. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol. |
Sanitization | Sanitization using NaOH reduces microbial contamination of the resin bed to a minimum without dismantling the column. The CIP procedures recommended above also sanitize Sepharose Fast Flow resins effectively. A concentration of 1.0 to 2.0 M NaOH with a contact time of 30 to 60 min has proved effective for most microbial contamination. |
Pack size | 2.5 L |
Dimensions | 126 × 200 mm |
Column volume | 2.5 L |
Column i.d. | 126 mm |
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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