ReadyToProcess Sepharose 6 FF

• Cat#No#: Re-317P
• Product Overview: ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. These ReadyToProcess columns are prepacked with Sepharose 6 Fast Flow chromatography resin for industrial processing at high flow rates and moderate pressures.
• Description: Sepharose 6 Fast Flow size exclusion chromatography resin is based on cross-linked 6% agarose matrix, which gives good physical stability and chromatographic qualities. The matrix was developed specifically to meet the high-throughput demands of industrial process separations. The rigidity permits high flow rates, which in turn gives good resolution in a minimum amount of time. Sepharose Fast Flow matrices enable the rapid processing of large volumes making these resins an excellent choice for use at process scale.
• Characteristic: Industrial-scale separation of large molecules and virus particles.
  High flow velocities at moderate pressures deliver good resolution with speed.
  Low non-specific interactions and excellent recoveries.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 1.2 bar (0.12 MPa, 17 psi)
• Matrix: Cross-linked agarose, 6%, spherical
• Average particle size: ~ 90 µm
• Recommended column height: 200 mm
• Chemical stability: Stable to commonly used aqueous buffers, 2.0 M NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 2% asepto, 6 M guanidine hydrochloride, 8 M urea.
• pH working range: 3 to 13
• CIP stability: 2 to 14
• Autoclavable: 20 min at 121°C in H2 O
• Storage: 4°C to 30°C, 20% ethanol
• Cleaning-in-place: Remove precipitated proteins: Wash the column with 4 bed volumes of 1.0 to 2.0 M NaOH solution at 40 cm/h, followed by 2 to 3 bed volumes of water.
  Remove strongly bound hydrophobic proteins, lipoproteins, and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol (apply gradients to avoid air bubble formation when using high concentrations of organic solvents). Alternatively, wash the column with detergent in a basic or acidic solution. For example, use 0.5% nonionic detergent in 1.0 M acetic acid. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
• Sanitization: Sanitization using NaOH reduces microbial contamination of the resin bed to a minimum without dismantling the column. The CIP procedures recommended above also sanitize Sepharose Fast Flow resins effectively. A concentration of 1.0 to 2.0 M NaOH with a contact time of 30 to 60 min has proved effective for most microbial contamination.
• Pack size: 2.5 L
• Dimensions: 126 × 200 mm
• Column volume: 2.5 L
• Column i.d.: 126 mm