RealBand 3-color Broad Range Protein Marker

• Cat#No#: MAR-038E
• Product Overview: This product is a ready-to-use three-color protein standard, containing 13 pre-stained proteins, covering various molecular weights from 5 to 245 kDa in Tris-Glycine buffer (in Bis-Tris (MOPS) buffer 3.5 to 235 kDa in solution and Bis-Tris (MES) buffer). Proteins are covalently coupled to different chromophores to facilitate identification of bands. When separated on SDS-PAGE (Tris-Glycine buffer), the two reference proteins carry enhanced intensity bands corresponding to 25 kDa in green and 75 kDa in red, respectively.
• Form: Each protein in the buffer is about 0.1~0.4 mg/ml. The buffer is 20 mM Tris-phosphate, pH 7.5, 25°C, 2% SDS, 0.2 mM dithiothreitol, 3.6 M urea and 15% (v/v) glycerol.
• Applications: Used to monitor protein separation during SDS-PAGE, verify the Western transfer efficiency on the membrane (PVDF, nylon or nitrocellulose) and estimate the size of the protein
• Storage: 4°C ≥ 3 months; -20°C ≥ 24 months
• Quality control analysis: 13 protein bands were formed in 15% SDS-PAGE (Bis-Tris gel, MES buffer), which were transferred to NC membrane by Western blot.
• Loading volume: 15-well or 10-well micro-gels can be clearly seen by loading 3μl or 5μl electrophoresis respectively.The Western membrane transfer efficiency test is generally 1.5~2.5 μl.