Product Information | |
Cat#No# | MAR-037E |
Product Overview | This product is a ready-to-use three-color protein standard, containing 10 kinds of pre-stained proteins, covering various molecular weights from 10 to 180 kDa (9 to 170 kDa in Bis-Tris (MOPS)). 10 to 170kDa in Tris (MES) buffer). When separated on SDS-PAGE (Tris-Glycine buffer), the protein is covalently coupled to the blue chromophore, except for the two reference bands (one green band and one red band at 25kDa and 75kDa, respectively) ) |
Form | 1 ml buffer contains approximately 0.1, 0.4 mg of protein, 2% SDS, 0.2 mM DTT, 3.6 M urea and 15% (v/v) glycerol. |
Applications | Used to monitor protein separation during SDS-PAGE, verify the Western transfer efficiency on the membrane (PVDF, nylon or nitrocellulose) and estimate the size of the protein |
Storage | 4°C ≥ 3 months; -20°C ≥ 24 months |
Quality control analysis | Under normal conditions, the 3-color Regular Range Protein Marker showed 10 bands (Tris-glycine buffer) on a 15% SDS-PAGE gel and subsequent immunoblotting to a nitrocellulose membrane. |
Loading volume | 15-well or 10-well micro-gels can be clearly seen by loading 3μl or 5μl electrophoresis respectively.The Western membrane transfer efficiency test is generally 1.5~2.5 μl. |
Notes | 5 μl each time can be used 50 times. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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