Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack of 5 Columns

• Cat#No#: AC-021A
• Common Name: Protein A
• Product Overview: recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
  Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.
  The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.
  Enabled by proprietary technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.
  Each gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.
• Characteristic: High capacity, > 40 mg human IgG per column
  High selectivity for high purity
  Minimal ligand leakage, can be reused
• Column: polypropylene (body)
  Low density polyethylene (top snap cap and bottom tip cap)
• Matrix: 4% agarose
• Average particle size: 45 μm - 165 μm, with an average size of ~ 90 μm
• Ligand: Recombinant protein A produced in E.coli
• Dynamic binding capacity: >40 mg human IgG
• Recommended flow rate: Gravity flow, 100 - 300 cm/hour
• Maximum Pressure Drop: 0.1 MPa (1 bar)
• Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
• pH working range: Long term: pH3 - 9; Short term: pH2 - 10
• Storage: 2 - 8°C, storage buffer: 20% ethanol