Recombinant Trypsin 3.0 (MS Grade)

• Sizes: 20 μg
• Description: rTrypsin 3.0 is a cutting-edge recombinant protease engineered with a unique gene sequence and methylated lysines. It provides rapid protein hydrolysis with zero self-degradation. Designed for high-performance applications, it is ideal for single-cell proteomics, large-scale cohort studies, and detailed protein characterization.
• Physical Appearance: Lyophilized powder with 20 μg Trehalose
• Molecular Weight: 23.7 kDa
• Resuspension Buffer: Achieve a 0.5 μg/μL concentration by dissolving in 40 μL of 50 mM acetic acid.
• Storage: Preserve the lyophilized solid at –20 °C; store the active enzyme at –80 °C after reconstitution.
• Shelf life: Store at -20 ℃ for up to 24 months.
• Stability: The rTrypsin 3.0 enzyme functions optimally at pH values ranging from 7.0 to 9.0.
• Usage Notes: 1. Compatible buffers: 50 mM ABC, 20 mM Tris, or HEPES (pH 7.0–9.0). 2. Digestion parameters: Use a 1:50 enzyme-to-substrate ratio at a concentration of 0.5 µg/µL. Process in a dry bath at 37 °C for 30 minutes.
• Activity: rTrypsin: 15,000 units/mg.
• Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1.0 μmol of BAEE per minute at 25°C and pH 7.8, measured at an absorbance of 253 nm.