rGlu-C Endopeptidase, MS Grade

• Sizes: 50μg
• Description: A recombinant serine protease, rGlu-C precisely hydrolyzes peptide bonds at the C-terminus of glutamic acid (Glu). Its site-selectivity is buffer-dependent: it exhibits high specificity for glutamic acid in ammonium acetate or ammonium bicarbonate, while also cleaves at aspartic acid (Asp) in phosphate buffers. It functions optimally within a pH range of 4.0 to 9.0.
• Physical Appearance: Lyophilized in the presence of trehalose (1.65 mg/vial)
• Molecular Weight: 24 kDa
• Resuspension Buffer: Use double-distilled water (not provided) to reconstitute the rGlu-C powder.
• Storage: Freeze-dried powder should be kept at –4 °C to -20 °C; reconstituted solution remains stable at -20 °C for 8 weeks.
• Shelf life: Store at -20 ℃ for up to 36 months.
• Stability: Shows highest enzymatic activity within a pH window of 4.0 to 9.0.
• Usage Notes: Reconstitute MS-grade rGlu-C with double-distilled water. Before adding the enzyme, prepare protein substrates in Tris-HCl (pH 8.0) at a 50 mM concentration. Employ a protein-to-protease ratio of 20:1 (w/w). Once mixed, maintain the reaction at 37 °C for a 4-hour incubation period.
• Activity: 800 U/min/mg.