rGlu C Protease, MS Grade

• sizes: 50μg
• Description: Mass Spec Grade Endoproteinase rGlu-C is a serine protease that specifically cleaves peptide bonds at the C-terminus of glutamic acid residues. Specificity for glutamic acid is high in ammonium bicarbonate and ammonium acetate buffers. In phosphate buffers, cleavage occurs at both aspartic and glutamic acid residues. rGlu-C activity is optimal in the pH range of 4.0 – 9.0. This mass spec grade enzyme can be used alone or in combination with other proteases to generate protein digests.
• Physical Appearance: Lyophilized in the presence of trehalose (1.65 mg/vial)
• Molecular Weight: 24 kDa
• Resuspension Buffer: Resuspend rGlu-C powder in user-supplied double-distilled water.
• Storage: Store the lyophilized powder at –4 °C or -20 °C; store reconstituted enzyme solution at -20 °C for up to 8 weeks.
• Shelf life: 36 months at -20 ℃
• Stability: Maximally active in the pH range of 4-9
• Usage Notes: For maximum activity, resuspend Mass Spectrum Grade rGlu-C with user-supplied double-distilled water. Dissolve protein samples with Tris-HCl (pH 8) buffer to a final protein concentration of 50 mM prior to digestion. Add rGlu-C protease at a protein:protease ratio of 20:1 (w/w). Mix well and incubate at 37 °C for 4 h.
• Activity: 800 U/min/mg.