rLys C Protease, MS Grade

• sizes: 20 μg
• Description: Mass Spec Grade rLys-C specifically hydrolyzes peptide bonds at the carboxyl side of lysine residues. An 8 M urea solution is recommended for denaturation. rLys-C efficiently cleaves difficult-to-digest proteins because it retains activity under denaturing conditions.
• Physical Appearance: Lyophilized powder
• Molecular Weight: 27 kDa
• Resuspension Buffer: rLys C Resuspension Buffer is composed of 50 mM Acetic acid buffer.
• Storage: Store the lyophilized powder at –20 °C; store reconstituted enzyme at –80 °C.
• Shelf life: 24 months at -20 ℃
• Stability: rLys-C is maximally active at pH 8 and reversibly inactivated at pH 3.
• Usage Notes: For maximum activity, resuspend Mass Spectrum Grade rLys-C in the provided resuspension buffer. Recommend using 6 - 8 M urea and 50 mM Tris-HCl (pH 8) to denature protein or protein mixtures prior to digestion. Add rLys-C to the mixture to reach a protein:protease ratio of 25:1 (w/w). Mix well and incubate at 37 °C for 3 - 4 hours.
• Activity: 25 units/min/mg