rProtein A Agarose 4 Fast Flow
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rProtein A Agarose 4 Fast Flow

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AC-013A rProtein A Agarose 4 Fast Flow 5ml Inquiry
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Product Information
Cat#No# AC-013A
Common Name Protein A
Product Overview rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.

The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.

Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.
Characteristic High capacity, > 40 mg human IgG / mL of settled resins
High selectivity for high purity
Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
Minimal ligand leakage, can be reused
Versatile, ready-to-use with convenient formats
Matrix Cross-linked 4% agarose supplied as a 50% slurry
Average particle size 45 μm - 165 μm
Ligand Recombinant protein A with Ig binding domains
Dynamic binding capacity >40 mg human IgG/mL medium
Recommended flow rate Gravity flow and medium pressure, 30 - 300 cm/hour
Maximum Pressure Drop 0.3 MPa (3 bar)
Chemical stability All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
pH working range Long term: pH2 - 9; Short term: pH2 - 11
Storage 2 - 8°C, storage buffer: 20% ethanol
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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