rProtein A Agarose 4 Fast Flow

• Cat#No#: AC-013A
• Common Name: Protein A
• Product Overview: rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
  Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.
  
  The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.
  
  Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.
• Characteristic: High capacity, > 40 mg human IgG / mL of settled resins
  High selectivity for high purity
  Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
  Minimal ligand leakage, can be reused
  Versatile, ready-to-use with convenient formats
• Matrix: Cross-linked 4% agarose supplied as a 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Recombinant protein A with Ig binding domains
• Dynamic binding capacity: >40 mg human IgG/mL medium
• Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
• pH working range: Long term: pH2 - 9; Short term: pH2 - 11
• Storage: 2 - 8°C, storage buffer: 20% ethanol