rProtein A Agarose 4FF, Prepacked Cartridge
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rProtein A Agarose 4FF, Prepacked Cartridge

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AC-018A rProtein A Agarose 4FF, Prepacked Cartridge 5x1ml Inquiry
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Product Information
Cat#No# AC-018A
Common Name Protein A
Product Overview rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in E. coli and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.

Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.
Characteristic High capacity, > 40 mg human IgG / mL of settled resins
High selectivity for high purity
Minimal ligand leakage, can be reused
Ready-to-use, compatible with standard automated systems
Matrix Highly cross-linked 4% agarose
Average particle size 45 μm - 165 μm, with an average of ~ 90 μm
Ligand Recombinant protein A produced in E.coli
Dynamic binding capacity >40 mg human IgG/mL medium
Recommended flow rate Gravity flow and medium pressure, 30 - 300 cm/hour
Maximum Pressure Drop 0.3 MPa (3 bar)
Chemical stability All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
pH working range Long term: pH3 - 10; Short term: pH2 - 11
Storage 2 - 8°C, storage buffer: 20% ethanol
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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