Product Information | |
Cat#No# | AC-018A |
Common Name | Protein A |
Product Overview | rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in E. coli and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit. Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications. |
Characteristic | High capacity, > 40 mg human IgG / mL of settled resins High selectivity for high purity Minimal ligand leakage, can be reused Ready-to-use, compatible with standard automated systems |
Matrix | Highly cross-linked 4% agarose |
Average particle size | 45 μm - 165 μm, with an average of ~ 90 μm |
Ligand | Recombinant protein A produced in E.coli |
Dynamic binding capacity | >40 mg human IgG/mL medium |
Recommended flow rate | Gravity flow and medium pressure, 30 - 300 cm/hour |
Maximum Pressure Drop | 0.3 MPa (3 bar) |
Chemical stability | All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. |
pH working range | Long term: pH3 - 10; Short term: pH2 - 11 |
Storage | 2 - 8°C, storage buffer: 20% ethanol |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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