Cat# | Product Name | Size | Price | Qty | Inquiry |
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AC-010A | rProtein A Agarose, Settled Resin | 5ml | Inquiry | ||
25ml | Inquiry | ||||
100ml | Inquiry | ||||
200ml | Inquiry |
Product Information | |
Cat#No# | AC-010A |
Common Name | Protein A |
Product Overview | rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E.coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit. Enabled by proprietary technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications. |
Characteristic | High capacity, > 40 mg human IgG / mL of settled resins High selectivity for high purity Minimal ligand leakage, can be reused |
Matrix | 4% agarose supplied as 50% slurry |
Average particle size | 45 μm - 165 μm |
Ligand | Recombinant protein A produced in E.coli |
Dynamic binding capacity | >40 mg human IgG/mL medium |
Recommended flow rate | Gravity flow, 100 - 300 cm/hour |
Maximum Pressure Drop | 0.1 MPa (1 bar) |
Chemical stability | All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. |
pH working range | Long term: pH3 - 9; Short term: pH2 - 10 |
Storage | 2 - 8°C, storage buffer: 20% ethanol |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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