rProtein A Magnetic Agarose, Settled Resin

• Cat#No#: ACH-002M
• Common Name: Protein A
• Product Overview: rProtein A Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
  Proprietary MagIndex technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.
  The superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.
  Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.
  The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.
• Characteristic: High capacity, > 10 mg human IgG / mL of settled resins
  High selectivity for high purity
• Applications: Quick isolation of antibodies from sera and ascites fluids
  Purification of scFc fragment from cell cultures
  Depletion of unwanted antibodies and fragments from sample
• Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry
• Average particle size: 30 μm - 100 μm
• Ligand: Recombinant protein A containing only Ig binding domains
• Dynamic binding capacity: >10 mg human IgG/mL medium
• pH working range: Long term: pH2 - 9; Short term: pH2 - 11
• Storage: 2 - 8°C, storage buffer: PBS containing 0.01% Tween-20 and 0.02% NaN3