rProtein A Sepharose Fast Flow antibody purification resin

• Cat#No#: rP-339C
• Product Overview: rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.
  For monoclonal and polyclonal antibody purification at both laboratory and process scale.
  Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
  Free from animal-derived components.
  Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: The recombinant Protein A used in the manufacture of rProtein A Sepharose Fast Flow is specially engineered to give very high binding capacities. It is produced in E. coli and purified by a multi-step chromatographic procedure. The purification does not involve the use of IgG or any other proteins. The purified recombinant Protein A is tested according to established specifications before being released for the manufacture of rProtein A Sepharose Fast Flow.
• Characteristic: High dynamic binding capacity for monoclonal antibodies, with high recovery and high purity.
  No mammalian components involved during the manufacturing process.
  Easy to scale up.
• Maximum operating pressure: 150–250 cm/h at 0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
• Ligand Coupling Method: Epoxy activation
• Matrix: Cross-linked agarose, 4%, spherical
• Particle Size: 60 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Recombinant protein A (E.coli)
• Ligand density: 6 mg rProtein A/ml medium
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~35 mg hIgG/mL resin
• Recommended flow rate: 150–250 cm/h at 0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water.
• Recommended column height: 25 cm
• Chemical stability: Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol.
• pH working range: 3–10
• CIP stability: 3–12
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Reducing agent, for example, 100 mM 1-Thioglycerol followed by 15 mM NaOH is among the most efficient cleaning protocols for rProtein A Sepharose Fast Flow. As an alternative cleaning protocol 6 M guanidine hydrochloride can be used. To remove hydrophobically bound substances a solution of non-ionic detergent or ethanol is recommended.
• Sanitization: For sanitizing rProtein A Sepharose Fast Flow, we recommend storage in a solution containing 0.1 M acetic acid/20% ethanol or 2% hibitane digluconate/20% ethanol.
• Pack size: 5 mL
• Maximum flow velocity: 250 cm/h
• Dimensions: 5 cm