rProtein A Seplife Suno is a Protein A affinity chromatography filler developed in response to the requirements of antibody customers. It uses spherical, highly crosslinked agarose gel as a matrix to bond alkaline resistant recombinant protein A to this matrix through independent innovation technology. It can be specifically bound to the Fc region of antibodies and used for the isolation and purification of antibodies (monoclonal antibody and polyclonal antibody). With one-step affinity chromatography, high purity antibodies can be obtained from ascites, serum or culture medium samples.
Appearance
Globular gel, odorless and tasteless
Matrix
Highly crosslinked agarose microspheres
Average particle size
65μm
Ligand
Alkali resistant recombinant Protein A
Ligand concentration
8~9mg/ml
Maximum backpressure
0.3MPa
Bulk density
70mg/ml
Recommended flow rate
>250cm/h(0.1MPa)
Chemical stability
0.1-0.5M NaOH
Storage
Keep sealed in a dry, ventilated and clean place at 4-8°C (20% ethanol storage solution), and do not freeze. The used columns are kept in 20% ethanol solution at 4-8°C.