rProtein A/G Agarose 4 Fast Flow

• Cat#No#: AC-016A
• Common Name: Protein A/G
• Product Overview: rProtein A/G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of a boarder range of immunoglobulin classes and subclasses. It is versatile, adaptable tool for antibody purification and immunoprecipitation.
  Recombinant fusion Protein A/G is a genetically engineered protein (MW ~ 50 kDa) that contains four Fc binding domains from Protein A and two from Protein G and therefore combines the IgG binding profiles of both Protein A and Protein G. The binding of rProtein A/G to immunoglobulins shows less pH-dependent than Protein A. In addition, unlike native Protein A and Protein G that contain albumin-recognition region and binding sites for other proteins, rProtein A/G has these non-immunoglobulin binding sites eliminated, showing super binding specificity for a variety of immunoglobulins.
  rProtein A/G Agarose 4FF has rProtein A/G immobolized onto highly cross-linked 4% agarose beads via a stable thioether linkage. The coupling technique is optimized to give a high binding capacity for IgG.
• Characteristic: 10 ~15 mg hIgG/mL resins
  Optimized for immunoprecipitation
  Binds to a broader range of Ig species
  Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
  Minimal ligand leakage, can be reused
  Versatile, ready-to-use with convenient formats
• Matrix: Highly cross-linked 4% agarose supplied as 50% slurry
• Average particle size: 45 μm - 165 μm, with an average of ~ 90 μm
• Ligand: Recombinant fusion protein A/G produced in E.coli
• Dynamic binding capacity: 10 ~ 15 mg human IgG/mL medium
• Recommended flow rate: Gravity flow, 100 - 300 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
• pH working range: pH3 - 10
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol