rProtein A/G Magnetic Agarose, Settled Resin

• Cat#No#: ACH-003M
• Common Name: Protein A/G
• Product Overview: rProtein A/G Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of a wide range of immunoglobulins, as well as Fc region-containing fusion proteins.
  Proprietary MagIndex technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.
  The superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.
  Superparamagnetic core with fast response to magnetic field
  Low nonspecific binding for assay accuracy
  High density of the Protein A/G ligand for optimal yield of antibodies and Ig fragments
  High stability in common buffer systems and detergents
  Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. While the native protein G has 3 IgG binding domains as well as sites for albumin and cell-surface binding, recombinant protein G has only Ig recognition domains but no other non-specific sequences.
  Recombinant Protein A/G is a recombinant chimeric protein that is composed of the IgG binding domains of both Protein A and Protein G. rProtein A/G contains five binding domains from Protein A and two from Protein G. The binding of rProtein A/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Therefore, rProtein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples.
• Characteristic: High capacity, > 10 mg human IgG / mL of settled resins
  High selectivity for high purity
• Applications: Quick isolation of antibodies from sera and ascites fluids
  Purification of recombinant antibody or scFc fragment from cell cultures
  Depletion of unwanted antibodies and fragments from sample
• Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry
• Average particle size: 30 μm - 100 μm
• Ligand: Recombinant chimeric protein A/G containing only Ig binding domains
• Dynamic binding capacity: >10 mg human IgG/mL medium
• pH working range: Long term: pH2 - 9; Short term: pH2 - 11
• Storage: 2 - 8°C, storage buffer: PBS containing 0.01% Tween-20 and 0.02% NaN3