rProtein G Agarose 4 Fast Flow

• Cat#No#: AC-015A
• Common Name: Protein G
• Product Overview: rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.
  Protein G is derived from Streptococcus strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.
  Showing super binding specificity for IgG species, rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.
• Characteristic: High capacity, > 30 mg human IgG / mL of settled resins
  High selectivity for high purity
  Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
  Minimal ligand leakage, can be reused
  Versatile, ready-to-use with convenient formats
• Matrix: Highly cross-linked 4% agarose supplied as 50% slurry
• Average particle size: 45 μm - 165 μm, with an average of ~ 90 μm
• Ligand: Recombinant protein G produced in E.coli
• Dynamic binding capacity: >30 mg human IgG/mL medium
• Recommended flow rate: Gravity flow, 100 - 300 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
• pH working range: Long term: pH3 - 9; Short term: pH2 - 10
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol