rProtein G Agarose 4 FF, Prepacked Cartridge
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rProtein G Agarose 4 FF, Prepacked Cartridge

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AC-019A rProtein G Agarose 4 FF, Prepacked Cartridge 5x1ml Inquiry
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Product Information
Cat#No# AC-019A
Common Name Protein G
Product Overview rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.
Protein G is derived from Streptococcus strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.
Showing super binding specificity for IgG species, rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.
Matrix Highly cross-linked 4% agarose
Average particle size 45 μm - 165 μm, with an average of ~ 90 μm
Ligand Recombinant protein G produced in E.coli
Dynamic binding capacity >30 mg human IgG/mL medium
Recommended flow rate Gravity flow, 100 - 300 cm/hour
Maximum Pressure Drop 0.3 MPa (3 bar)
Chemical stability All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
pH working range Long term: pH3 - 9; Short term: pH2 - 10
Storage 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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