rProtein L Agarose 4 Fast Flow

• Cat#No#: AC-017A
• Common Name: Protein L
• Product Overview: rProtein L Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, as well as Fab, scFv fragments of immunoglobulins from biological fluids and from cell culture media at both laboratory and process scale.
  Protein L is first isolated from the surface of bacterial species Peptostreptoccus magnus and now recombinant Protein L is obtained by gene recombination technology. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins, Protein L binds the variable region of antibodies kappa light chain and binds a wider range of Ig classes and subclasses. Protein L is therefore particular suitable for capture of a wide range of different-sized antibody fragments such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). It is also suitable for mouse IgM purification from cell.
  rProtein L Agarose 4 Fast Flow utilizes a stable thioether linkage between rProtein L and the cross-linked agarose beads and is optimized for high binding capacity for immunoglobulins.
• Matrix: Cross-linked 4% agarose supplied as a 50% slurry
• Average particle size: 45 μm - 165 μm, with an average of ~ 90 μm
• Ligand: Recombinant protein L produced in E.coli
• Dynamic binding capacity: >15 mg human IgG/mL medium
• Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
• Storage: 2 - 8°C, storage buffer: 20% ethanol